Biology

The correct answer is: D.It prevents the introduction of new alleles into the gene pool.

Explanation:

The assumption of no mutations in Hardy-Weinberg equilibrium limits evolution because mutations are the source of new genetic variation in a population. Without mutations, the gene pool remains static, and no new alleles are introduced. Since evolution depends on genetic variation for natural selection, genetic drift, and other processes to act upon, the lack of mutations prevents these evolutionary mechanisms from creating changes in allele frequencies.

Here’s why the other options are incorrect:

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