The correct answer is: B.Microtubule spindle.
Explanation:
During metaphase of mitosis, microtubules form the spindle apparatus that radiates out from the centrioles (or centrosomes) in the cytoplasm. These microtubules help align the chromosomes at the metaphase plate in preparation for their separation in the next stage of mitosis (anaphase).
A.Nuclear membrane: The nuclear membrane breaks down during prophase, so it is not involved in metaphase.
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