Question #6426853Single Choice
Biology
Question
A researcher performs a PCR reaction and observes weak amplification of the target DNA. Which of the following adjustments would MOST LIKELY improve the result?
Options
A
Increase the annealing temperature above the melting temperature (Tm) of the primers.
B
Add more template DNA to the reaction.
C
Optimize the magnesium ion (Mg²⁺) concentration.
D
Reduce the number of cycles in the reaction.
Answer & Analysis
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